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Cellular Morphogenesis and Membrane Trafficking
Research Assistant Professor, Pharmacological Sciences
Ph.D., Peking Union Medical College & Chinese Academy of Medical Sciences , China
Postdoctoral, SUNY Stony Brook
Our research aims to elucidate the molecular mechanisms that underlie cellular morphogenesis and membrane dynamics in mammalian cells. We are focusing on roles of phospholipids in actin cytoskeletal reorganization and membrane trafficking, and are exploring the regulatory circuitry that controls these processes. One of the ongoing projects is to examine how the dynamic changes of membrane phospholipid signaling regulate endocytosis of G protein-coupled receptors and receptor tyrosine kinases. The other is to determine how such signaling regulates cell adhesion, migration and polarity. Our long-term goal is to understand the mechanisms through which defects in these pathways contribute to the pathophysiology of human disease .
Selected Publications
Zhao C, Du G, Skowronek K, Frohman MA,
Bar-Sagi D. (2007). Phospholipase D2-generated phosphatidic acid couples EGFR
stimulation to Ras activation by Sos. Nat Cell Biol. 9(6):707-712.
Mor A, Campi G, Du G, Zheng Y, Foster
DA, Dustin ML, Philips MR. (2007). The lymphocyte function-associated antigen-1
receptor costimulates plasma membrane Ras via phospholipase D2. Nat Cell Biol.
9(6):713-9.
Du. G., Yonekubo, J., Zeng, Y.,
Osisami, M., and Frohman, M.A. (2006). Design of small-hairpin RNA expression
vectors based on miRNAs and RNA splicing. FEBS J. 273(23):5421.
LaLonde M, Janssens H, Yun S, Crosby
J, Redina O, Olive V, Altshuller YM, Choi SY, Du G, Gergen JP, Frohman MA.
(2006). A role for Phospholipase D in Drosophila embryonic cellularization. BMC
Dev Biol. 6:60.
Scotto-Lavino E, Du G, Frohman MA.
(2006). 5' end cDNA amplification using classic RACE. Nat Protoc.1(6):2555-62.
Scotto-Lavino E, Du G, Frohman MA.
(2006). 3' end cDNA amplification using classic RACE. Nat Protoc. 1(6):2742-5.
Scotto-Lavino E, Du G, Frohman MA.
(2006). Amplification of 5' end cDNA with 'new RACE'. Nat Protoc. 1(6):3056-61.
Corrotte M, Chasserot-Golaz S, Huang
P, Du G, Ktistakis NT, Frohman MA, Vitale N, Bader MF, and Grant NJ. (2006).
Dynamics and function of phospholipase D and phosphatidic acid during
phagocytosis. Traffic. 7(3):365-77.
Cai
D, Netzer WJ, Zhong M, Lin Y, Du G, Frohman M, Foster DA, Sisodia SS, Xu
H, Gorelick FS and Greengard P. (2006). Presenilin-1 (PS1) utilizes
phospholipase D1 as a negative regulator of b-amyloid formation. Proc Natl
Acad Sci USA. 103(6):1941-6.
Su W, Chardin P, Yamazaki M, Kanaho Y, Du G (2006). RhoA-mediated
Phospholipase D1 signaling is not required for the formation of stress
fibers and focal adhesions. 18(4) 469-478.
Corrotte M, Chasserot-Golaz S, Huang P, Du G, Ktistakis NT,
Frohman MA, Vitale N, Bader MF, Grant NJ (2006). Dynamics
and function of phospholipase d and phosphatidic Acid during
phagocytosis. Traffic 7 365-77.
Cai D, Netzer WJ, Zhong M, Lin Y, Du G, Frohman M, Foster DA,
Sisodia SS, Xu H, Gorelick FS, Greengard P (2006). Presenilin-1
uses phospholipase D1 as a negative regulator of beta-amyloid
formation. Proc Natl Acad Sci U S A. 103 1941-6.
Zhou Y*, Du G*, Hu X, Yu S, Liu Y, Xu Y, Huang X, Liu J, Yin B,
Fan M, Peng X, Qiang B, Yuan J. (2005). Nectin-like
molecule 1 is a protein 4.1N associated protein and recruits protein
4.1N from cytoplasm to the plasma membrane. 1669(2) 142-54. *equal contribution
Xiao N, Du G, Frohman MA (2005). Peroxiredoxin
II functions as a signal terminator for H2O2-activated phospholipase
D1. FEBS J. 272 3929-37.
Zheng XL, Gui Y, Du G, Frohman MA, Peng DQ (2004). Calphostin-C
induction of vascular smooth muscle cell apoptosis proceeds through
phospholipase D and microtubule inhibition. J Biol Chem. 279 7112-8.
Du G, Huang P, Liang BT, Frohman MA (2004). Phospholipase
D2 localizes to the plasma membrane and regulates angiotensin II
receptor endocytosis. Mol. Biol. Cell 15 1024-30.
Zhang Y, Huang P, Du G, Kanaho Y, Frohman MA, Tsirka SE (2004). Increased
expression of two phospholipase D isoforms during experimentally
induced hippocampal mossy fiber outgrowth. GLIA 46 74-83.
Du G, Altshuller YM, Vitale N, Huang P, Chasserot-Golaz S,
Morris AJ, Bader MF, Frohman MA (2003). Regulation
of phospholipase D1 subcellular cycling through coordination of
multiple membrane association motifs. J Cell Biol. 162 305-15.
Ballou LM, Jiang YP, Du G, Frohman MA, Lin RZ (2003). Ca(2+)-
and phospholipase D-dependent and -independent pathways activate mTOR
signaling. Federation of European Biochemical Societies 550 51-6.
Fang Y, Park IH, Wu AL, Du G, Huang P, Frohman MA, Walker SJ,
Brown HA, Chen J (2003). PLD1
regulates mTOR signaling and mediates Cdc42 activation of S6K1. Current Biology 13 2037-44.
Du G, Morris AJ, Sciorra VA, Frohman MA (2002). G-protein-coupled
receptor regulation of phospholipase D. Methods Enzymol. 345 265-74.
Humeau Y, Vitale N, Chasserot-Golaz S, Dupont JL, Du G, Frohman
MA, Bader MF, Poulain B (2001). A
role for phospholipase D1 in neurotransmitter release. Proc Natl Acad Sci U S A. 98 15300-5.
Vitale N, Caumont AS, Chasserot-Golaz S, Du G, Wu S, Sciorra
VA, Morris AJ, Frohman MA, Bader MF (2001). Phospholipase
D1: a key factor for the exocytotic machinery in neuroendocrine cells. EMBO J. 20 2424-34.
Wang J, Zhou Y, Yin B, Du G, Huang X, Li G, Shen Y, Yuan J,
Qiang B (2001). ASH2L:
alternative splicing and downregulation during induced megakaryocytic
differentiation of multipotential leukemia cell lines. Journal of molecular medicine 79 399-405.
Park JB, Kim JH, Kim Y, Ha SH, Yoo JS, Du G, Frohman MA, Suh
PG, Ryu SH (2000). Cardiac
phospholipase D2 localizes to sarcolemmal membranes and is inhibited by
alpha-actinin in an ADP-ribosylation factor-reversible manner. J Biol Chem. 275 21295-301.
Slaaby R, Du G, Altshuller YM, Frohman MA, Seedorf K (2000). Insulin-induced
phospholipase D1 and phospholipase D2 activity in human embryonic
kidney-293 cells mediated by the phospholipase C gamma and protein
kinase C alpha signalling cascade. Biochem J. 351 Pt 613-9.
Du G, Altshuller YM, Kim Y, Han JM, Ryu SH, Morris AJ, Frohman
MA (2000). Dual
requirement for rho and protein kinase C in direct activation of
phospholipase D1 through G protein-coupled receptor signaling. Mol. Biol. Cell 11 4359-68.
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