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Regulation of exocytosis, cell shape, and mitochondrial fusion by lipid signaling pathways
Professor, Pharmacological Sciences
Center for Molecular Medicine, room 438
M.D., Ph.D., University of Pennsylvania
Postdoctoral, University of California, San Francisco
michael@pharm.stonybrook.edu
2-1476
Selected Publications
Old Lab Page
New Lab Page
Our laboratory examines lipid signals that alter cell morphology, regulate mitochondrial fusion, and trigger subcellular trafficking of membrane vesicles during regulated exocytosis and endocytosis. Disease-related topics include neurodegenerative disease (Charcot-Marie Tooth syndrome), diabetes, cancer, and impaired phagocytic immune responses to pathogens.
The specific topic constitutes study of signaling pathways mediated by members of the superfamiles of enzymes known as Phospholipase D (PLD) and PI4P Kinase. Classic PLD is activated by a wide variety of agonists that signal through G-protein coupled or tyrosine kinase receptors. PLD has varied cellular roles including facilitating membrane vesicle trafficking and fusion of the vesicles into the plasma membrane during regulated exocytosis, and reorganizing the actin cytoskeleton. We are currently investigating roles for PLD and PI4P lipid kinase in actin cytoskeleton reorganization, myoblast differentiation and fusion, Glut-4 glucose transporter translocation, phagocytosis, and regulated exocytosis (insulin and histamine release). In addition, we are exploring a novel member of the PLD superfamily that localizes to mitochondria and regulates fusion, and may play a role in diabetes or cause neurodegenerative disease when dysfunctional.
Selected Publications
Cai D, Netzer WJ, Zhong M, Lin Y, Du G, Frohman M, Foster DA, Sisodia
SS, Xu H, Gorelick FS, Greengard P (2006). Presenilin-1
uses phospholipase D1 as a negative regulator of beta-amyloid
formation. Proc Natl Acad Sci U S A. 103 1941-6.
Corrotte M, Chasserot-Golaz S, Huang P, Du G, Ktistakis NT,
Frohman MA, Vitale N, Bader MF, Grant NJ (2006). Dynamics
and function of phospholipase D and phosphatidic acid during
phagocytosis. Traffic 7 365-77.
Choi SY, Gonzalvez F, Jenkins GM, Slomianny C, Chretien D,
Arnoult D, Petit PX, Frohman MA (2006). Cardiolipin
deficiency releases cytochrome c from the inner mitochondrial membrane
and accelerates stimuli-elicited apoptosis. Cell Death Differ. .
Suzuki T, Kanai Y, Hara T, Sasaki J, Sasaki T, Kohara M,
Maehama T, Taya C, Shitara H, Yonekawa H, Frohman MA, Yokozeki T,
Kanaho Y (2006). Crucial
role of the small GTPase ARF6 in hepatic cord formation during liver
development. Mol Cell Biol. 26 6149-56.
Huang P, Jiang Z, Teng S, Wong YC, Frohman MA, Chung SK, Chung
SS (2006). Synergism
between phospholipase D2 and sorbitol accumulation in diabetic cataract
formation through modulation of Na,K-ATPase activity and osmotic
stress. Exp Eye Res .
Andersson L, Bostr�m P, Ericson J, Rutberg M, Magnusson B,
Marchesan D, Ruiz M, Asp L, Huang P, Frohman MA, Bor�n J, Olofsson SO (2006). PLD1
and ERK2 regulate cytosolic lipid droplet formation. J Cell Sci. 119 2246-57.
Jenkins GM, Frohman MA (2005). Phospholipase
D: a lipid centric review. Cell Mol Life Sci. 62 2305-16.
Bader, M.-F. and Frohman, M.A. (2005). Phospholipase
D : Membrane traffic and Exocytosis In pres .
Singh AT, Frohman MA, Stern PH (2005). Parathyroid
hormone stimulates phosphatidylethanolamine hydrolysis by phospholipase
D in osteoblastic cells. Lipids(2) 40 1135-40.
Bostr�m P, Rutberg M, Ericsson J, Holmdahl P, Andersson L,
Frohman MA, Bor�n J, Olofsson SO (2005). Cytosolic
lipid droplets increase in size by microtubule-dependent complex
formation. Lipids 25 1945-51.
Xiao N, Du G, Frohman MA (2005). Peroxiredoxin
II functions as a signal terminator for H2O2-activated phospholipase
D1. FEBS J. 272 3929-37.
LaLonde MM, Janssens H, Rosenbaum E, Choi SY, Gergen JP, Colley
NJ, Stark WS, Frohman MA (2005). Regulation
of phototransduction responsiveness and retinal degeneration by a
phospholipase D-generated signaling lipid. J Cell Biol. 169 471-9.
Huang P, Altshuller YM, Hou JC, Pessin JE, Frohman MA (2005). Insulin-stimulated
plasma membrane fusion of Glut4 glucose transporter-containing vesicles
is regulated by phospholipase D1. Mol. Biol. Cell 16 2614-23.
Sasaki J, Sasaki T, Yamazaki M, Matsuoka K, Taya C, Shitara H,
Takasuga S, Nishio M, Mizuno K, Wada T, Miyazaki H, Watanabe H, Iizuka
R, Kubo S, Murata S, Chiba T, Maehama T, Hamada K, Kishimoto H, Frohman
MA, Tanaka K, Penninger JM, Yonekawa H, Suzuki A, (2005). Regulation
of anaphylactic responses by phosphatidylinositol phosphate kinase type
I {alpha}. J Exp Med. 201 859-70.
Zhang Y, Kanaho Y, Frohman MA, Tsirka SE (2005). Phospholipase
D1-promoted release of tissue plasminogen activator facilitates neurite
outgrowth. J Neurosci. 25 1797-805.
Watanabe H, Yokozeki T, Yamazaki M, Miyazaki H, Sasaki T,
Maehama T, Itoh K, Frohman MA, Kanaho Y (2004). Essential
role for phospholipase D2 activation downstream of ERK MAP kinase in
nerve growth factor-stimulated neurite outgrowth from PC12 cells. J Biol Chem. 279 37870-7.
Hughes WE, Elgundi Z, Huang P, Frohman MA, Biden TJ (2004). Phospholipase
D1 regulates secretagogue-stimulated insulin release in pancreatic
beta-cells. J Biol Chem. 279 27534-41.
Watanabe H, Yamazaki M, Miyazaki H, Arikawa C, Itoh K, Sasaki
T, Maehama T, Frohman MA, Kanaho Y (2004). Phospholipase
D2 functions as a downstream signaling molecule of MAP kinase pathway
in L1-stimulated neurite outgrowth of cerebellar granule neurons. J Neurochem. 89 142-51.
Zhang Y, Huang P, Du G, Kanaho Y, Frohman MA, Tsirka SE (2004). Increased
expression of two phospholipase D isoforms during experimentally
induced hippocampal mossy fiber outgrowth. GLIA 46 74-83.
Du G, Huang P, Liang BT, Frohman MA (2004). Phospholipase
D2 localizes to the plasma membrane and regulates angiotensin II
receptor endocytosis. Mol. Biol. Cell 15 1024-30.
Zheng XL, Gui Y, Du G, Frohman MA, Peng DQ (2004). Calphostin-C
induction of vascular smooth muscle cell apoptosis proceeds through
phospholipase D and microtubule inhibition. J Biol Chem. 279 7112-8.
LaLonde, M. and Frohman, M.A. (2004). Phospholipase
D. 3 306-313.
Du G, Altshuller YM, Vitale N, Huang P, Chasserot-Golaz S,
Morris AJ, Bader MF, Frohman MA (2003). Regulation
of phospholipase D1 subcellular cycling through coordination of
multiple membrane association motifs. J Cell Biol. 162 305-15.
Huang P, Frohman MA (2003). The
role of phospholipase D in Glut-4 translocation. Diabetes Metab Res Rev. BMC Neurosci. 19 456-63.
Frohman, M.A. (2003). Rapid
Amplification of cDNA Ends. In press.
Fang Y, Park IH, Wu AL, Du G, Huang P, Frohman MA, Walker SJ,
Brown HA, Chen J (2003). PLD1
regulates mTOR signaling and mediates Cdc42 activation of S6K1. Current Biology 13 2037-44.
Ballou LM, Jiang YP, Du G, Frohman MA, Lin RZ (2003). Ca(2+)-
and phospholipase D-dependent and -independent pathways activate mTOR
signaling. Federation of European Biochemical Societies 550 51-6.
Du G, Morris AJ, Sciorra VA, Frohman MA (2002). G-protein-coupled
receptor regulation of phospholipase D. Methods Enzymol. 345 265-74.
Yamazaki M, Miyazaki H, Watanabe H, Sasaki T, Maehama T,
Frohman MA, Kanaho Y (2002). Phosphatidylinositol
4-phosphate 5-kinase is essential for ROCK-mediated neurite remodeling.
J Biol Chem. 277 17226-30.
Choi WS, Kim YM, Combs C, Frohman MA, Beaven MA (2002). Phospholipases
D1 and D2 regulate different phases of exocytosis in mast cells. J Immunol. 168 5682-9.
Vitale N, Chasserot-Golaz S, Bailly Y, Morinaga N, Frohman MA,
Bader MF (2002). Calcium-regulated
exocytosis of dense-core vesicles requires the activation of
ADP-ribosylation factor (ARF)6 by ARF nucleotide binding site opener at
the plasma membrane. J Cell Biol. 159 79-89.
Du., G., Frohman, M.A. and Morris, A.J. (2001). Phospholipase
D: Isoforms and Function. 41 In press.
Vitale N, Caumont AS, Chasserot-Golaz S, Du G, Wu S, Sciorra
VA, Morris AJ, Frohman MA, Bader MF (2001). Phospholipase
D1: a key factor for the exocytotic machinery in neuroendocrine cells. EMBO J. 20 2424-34.
Freyberg Z, Sweeney D, Siddhanta A, Bourgoin S, Frohman M,
Shields D (2001). Intracellular
localization of phospholipase D1 in mammalian cells. Mol. Biol. Cell 12 943-55.
Frohman, M.A. and Engebrecht, J.E. (2001).
Phospholipase D in cell signaling. Chapter 15.
Zhao D, Frohman MA, Blusztajn JK (2001). Generation
of choline for acetylcholine synthesis by phospholipase D isoforms. 2 16.
Humeau Y, Vitale N, Chasserot-Golaz S, Dupont JL, Du G, Frohman
MA, Bader MF, Poulain B (2001). A
role for phospholipase D1 in neurotransmitter release. Proc Natl Acad Sci U S A. 98 15300-5.
Denmat-Ouisse LA, Phebidias C, Honkavaara P, Robin P, Geny B,
Min do S, Bourgoin S, Frohman MA, Raymond MN (2001). Regulation
of constitutive protein transit by phospholipase D in HT29-cl19A cells.
J Biol Chem. 276 48840-6.
Sciorra, V.A., Frohman, M.A., and Morris, A.J. (2000). Regulation
of Phospholipase D signaling by phosphoinositides. 269-297.
Wu YP, Siao CJ, Lu W, Sung TC, Frohman MA, Milev P, Bugge TH,
Degen JL, Levine JM, Margolis RU, Tsirka SE (2000). The
tissue plasminogen activator (tPA)/plasmin extracellular proteolytic
system regulates seizure-induced hippocampal mossy fiber outgrowth
through a proteoglycan substrate. J Cell Biol. 148 1295-304.
Slaaby R, Du G, Altshuller YM, Frohman MA, Seedorf K (2000). Insulin-induced
phospholipase D1 and phospholipase D2 activity in human embryonic
kidney-293 cells mediated by the phospholipase C gamma and protein
kinase C alpha signalling cascade. Biochem J. 351 Pt 613-9.
Lee C, Kim SR, Chung JK, Frohman MA, Kilimann MW, Rhee SG (2000). Inhibition
of phospholipase D by amphiphysins. J Biol Chem. 275 18751-8.
Frohman, M.A. and Tsirka, S.E. (2000). Polymerase
Chain reaction: specialized applications. Nature Encylopedia of Life
Sciences (Robertson, S., Editor). Macmillan, London. In press.
Zhang Y, Redina O, Altshuller YM, Yamazaki M, Ramos J,
Chneiweiss H, Kanaho Y, Frohman MA (2000). Regulation
of expression of phospholipase D1 and D2 by PEA-15, a novel protein
that interacts with them. J Biol Chem. 275 35224-32.
Du G, Altshuller YM, Kim Y, Han JM, Ryu SH, Morris AJ, Frohman
MA (2000). Dual
requirement for rho and protein kinase C in direct activation of
phospholipase D1 through G protein-coupled receptor signaling. Mol. Biol. Cell 11 4359-68.
Park JB, Kim JH, Kim Y, Ha SH, Yoo JS, Du G, Frohman MA, Suh
PG, Ryu SH (2000). Cardiac
phospholipase D2 localizes to sarcolemmal membranes and is inhibited by
alpha-actinin in an ADP-ribosylation factor-reversible manner. J Biol Chem. 275 21295-301.
Frohman MA, Kanaho Y, Zhang Y, Morris AJ (2000). Regulation
of phospholipase D1 activity by Rho GTPases. Methods Enzymol. 325 177-89.
Frohman MA, Morris AJ (1999). Phospholipase
D structure and regulation. Chem Phys Lipids. 98 127-40.
Frohman MA, Sung TC, Morris AJ (1999). Mammalian
phospholipase D structure and regulation. Biochim Biophys Acta. 1439 175-86.
Sung TC, Altshuller YM, Morris AJ, Frohman MA (1999). Molecular
analysis of mammalian phospholipase D2. J Biol Chem. 274 494-502.
Sung TC, Zhang Y, Morris AJ, Frohman MA (1999). Structural
analysis of human phospholipase D1. J Biol Chem. 274 3659-66.
Zhang, Y. and Frohman, M.A. (1999). Rapid
Amplification of cDNA Ends (RACE) to obtain full length cDNAs. 81-106.
Rizzo MA, Shome K, Vasudevan C, Stolz DB, Sung TC, Frohman MA,
Watkins SC, Romero G (1999). Phospholipase
D and its product, phosphatidic acid, mediate agonist-dependent raf-1
translocation to the plasma membrane and the activation of the
mitogen-activated protein kinase pathway. J Biol Chem. 274 1131-9.
Yamazaki M, Zhang Y, Watanabe H, Yokozeki T, Ohno S, Kaibuchi
K, Shibata H, Mukai H, Ono Y, Frohman MA, Kanaho Y (1999). Interaction
of the small G protein RhoA with the C terminus of human phospholipase
D1. J Biol Chem. 274 6035-8.
Sciorra VA, Rudge SA, Prestwich GD, Frohman MA, Engebrecht J,
Morris AJ (1999). Identification
of a phosphoinositide binding motif that mediates activation of
mammalian and yeast phospholipase D isoenzymes. EMBO J. 18 5911-21.
Zhang Y, Altshuller YM, Hammond SM, Hayes F, Morris AJ, Frohman
MA (1999). Loss
of receptor regulation by a phospholipase D1 mutant unresponsive to
protein kinase C. EMBO J. 18 6339-48.
Honda A, Nogami M, Yokozeki T, Yamazaki M, Nakamura H, Watanabe
H, Kawamoto K, Nakayama K, Morris AJ, Frohman MA, Kanaho Y (1999). Phosphatidylinositol
4-phosphate 5-kinase alpha is a downstream effector of the small G
protein ARF6 in membrane ruffle formation. Cell (Cambridge) 99 521-32.
Frohman, M.A. (1998). Molecular
Biology of Phospholipase D 65-70.
Nakashima S, Ohguchi K, Frohman MA, Nozawa Y (1998). Increased
mRNA expression of phospholipase D (PLD) isozymes during granulocytic
differentiation of HL60 cells. Biochim Biophys Acta. 1389 173-7.
Redina OE, Frohman MA (1998). Genomic
analysis of murine phospholipase D1 and comparison to phospholipase D2
reveals an unusual difference in gene size. Gene 222 53-60.
Redina OE, Frohman MA (1998). Organization
and alternative splicing of the murine phospholipase D2 gene. Biochem J. 331 ( P 845-51.
Slaaby R, Jensen T, Hansen HS, Frohman MA, Seedorf K (1998). PLD2
complexes with the EGF receptor and undergoes tyrosine phosphorylation
at a single site upon agonist stimulation. J Biol Chem. 273 33722-7.
Zhang Y, Frohman MA (1997). Using
rapid amplification of cDNA ends (RACE) to obtain full-length cDNAs. 69 61-87.
Hammond SM, Jenco JM, Nakashima S, Cadwallader K, Gu Q, Cook S,
Nozawa Y, Prestwich GD, Frohman MA, Morris AJ (1997). Characterization
of two alternately spliced forms of phospholipase D1. Activation of the
purified enzymes by phosphatidylinositol 4,5-bisphosphate,
ADP-ribosylation factor, and Rho family monomeric GTP-binding proteins
and protein kinase C-alpha. J Biol Chem. 272 3860-8.
Zhang, Y. and Frohman, M.A. (1997). Cloning
cDNA ends using RACE. 13 81-105.
Colley WC, Altshuller YM, Sue-Ling CK, Copeland NG, Gilbert DJ,
Jenkins NA, Branch KD, Tsirka SE, Bollag RJ, Bollag WB, Frohman MA (1997). Cloning
and expression analysis of murine phospholipase D1. Biochem J. 326 ( P 745-53.
Morris AJ, Frohman MA, Engebrecht J (1997). Measurement
of phospholipase D activity. Anal Biochem. 252 1-9.
Liang JO, Sung TC, Morris AJ, Frohman MA, Kornfeld S (1997). Different
domains of mammalian ADP-ribosylation factor 1 mediate interaction with
selected target proteins. J Biol Chem. 272 33001-8.
Sung, T.-C., Roper, K., Zhang, Y., Rudge, S., Temel, R.,
Hammond, S.M., Morris, A.J., Moss, B., Engebrecht, J., and Frohman,
M.A. (1997). )
Mutagenesis of Phospholipase D defines a superfamily including a
trans-Golgi viral protein required for poxvirus pathogenicity. 16 4519-4530.
Morris AJ, Hammond SM, Colley C, Sung TC, Jenco JM, Sciorra VA,
Rudge SA, Frohman MA (1997). Regulation
and functions of phospholipase D. 25 1151-7.
Colley WC, Sung TC, Roll R, Jenco J, Hammond SM, Altshuller Y,
Bar-Sagi D, Morris AJ, Frohman MA (1997). Phospholipase
D2, a distinct phospholipase D isoform with novel regulatory properties
that provokes cytoskeletal reorganization. Current Biology 7 191-201.
Luo JQ, Liu X, Hammond SM, Colley WC, Feig LA, Frohman MA,
Morris AJ, Foster DA (1997). RalA
interacts directly with the Arf-responsive, PIP2-dependent
phospholipase D1. Biochem Biophys Res Commun. 235 854-9.
Chen YG, Siddhanta A, Austin CD, Hammond SM, Sung TC, Frohman
MA, Morris AJ, Shields D (1997). Phospholipase
D stimulates release of nascent secretory vesicles from the trans-Golgi
network. J Cell Biol. 138 495-504.
Altshuller Y, Copeland NG, Gilbert DJ, Jenkins NA, Frohman MA (1996). Gcm1,
a mammalian homolog of Drosophila glial cells missing. Federation of European Biochemical Societies 393 201-4.
Morris AJ, Engebrecht J, Frohman MA (1996). Structure
and regulation of phospholipase D. 17 182-5.
Frohman MA, Morris AJ (1996). Rho
is only ARF the story. Phospholipid signalling. Current Biology 6 945-7.
Frohman, M. A. and Morris, A. J. (1996). Molecular
studies on phospholipase D genes: New twists on an old story. In
Lipases and transduction 87 250-258.
Chong JA, Tapia-Ram�rez J, Kim S, Toledo-Aral JJ, Zheng Y,
Boutros MC, Altshuller YM, Frohman MA, Kraner SD, Mandel G (1995). REST:
a mammalian silencer protein that restricts sodium channel gene
expression to neurons. Cell (Cambridge) 80 949-57.
Hammond SM, Altshuller YM, Sung TC, Rudge SA, Rose K,
Engebrecht J, Morris AJ, Frohman MA (1995). Human
ADP-ribosylation factor-activated phosphatidylcholine-specific
phospholipase D defines a new and highly conserved gene family. J Biol Chem. 270 29640-3.
Rose K, Rudge SA, Frohman MA, Morris AJ, Engebrecht J (1995). Phospholipase
D signaling is essential for meiosis. Proc Natl Acad Sci U S A. 92 12151-5.
Frohman, M.A. (1995). )
Rapid Amplification of cDNA Ends. 381-410.
Frohman MA (1994). On
beyond classic RACE (rapid amplification of cDNA ends). 4 S40-58.
Carroll PM, Tsirka SE, Richards WG, Frohman MA, Strickland S (1994). The
mouse tissue plasminogen activator gene 5' flanking region directs
appropriate expression in development and a seizure-enhanced response
in the CNS. 120 3173-83.
Frohman, M.A. (1994). )
Cloning PCR products: Strategies and tactics. In: PCR. The Polymerase
Chain Reaction. 14 - 37.
Frohman MA (1993). Rapid
amplification of complementary DNA ends for generation of full-length
complementary DNAs: thermal RACE. Methods Enzymol. 218 340-56.
Frohman MA, Martin GR, Cordes SP, Halamek LP, Barsh GS (1993). Altered
rhombomere-specific gene expression and hyoid bone differentiation in
the mouse segmentation mutant, kreisler (kr). 117 925-36.
Frohman, M.A., Dickinson, M.E., Hogan, B.L.M., and Martin, G.R.
(1993). Localization
of two new and related homeobox-containing genes to chromosomes 1 and
5, near the phenotypically similar mutant loci dominant hemimelia (Dh)
and hemimelic extra-toes (Hx). 91 :323-325..
Bulfone A, Puelles L, Porteus MH, Frohman MA, Martin GR,
Rubenstein JL (1993). Spatially
restricted expression of Dlx-1, Dlx-2 (Tes-1), Gbx-2, and Wnt-3 in the
embryonic day 12.5 mouse forebrain defines potential transverse and
longitudinal segmental boundaries. 13 3155-72.
Frohman MA, Martin GR (1992). Isolation
and analysis of embryonic expression of Hox-4.9, a member of the murine
labial-like gene family. 38 55-67.
Frohman M, Francfort JW, Cowing C (1991). T-dependent
destruction of thyroid isografts exposed to IFN-gamma. J Immunol. 146 2227-34.
Mizobuchi M, Frohman MA, Downs TR, Frohman LA (1991). Tissue-specific
transcription initiation and effects of growth hormone (GH) deficiency
on the regulation of mouse and rat GH-releasing hormone gene in
hypothalamus and placenta. Molecular endocrinology (Baltimore, Md.) 5 476-84.
Frohman, M.A. (). (1990). Rapid
Amplification of cDNA Ends (RACE): User Friendly cDNA Cloning. 5 11-15..
Frohman LA, Downs TR, Chomczynski P, Frohman MA (1990). Growth
hormone-releasing hormone: structure, gene expression and molecular
heterogeneity. 367 81-6.
Frohman MA, Boyle M, Martin GR (1990). Isolation
of the mouse Hox-2.9 gene; analysis of embryonic expression suggests
that positional information along the anterior-posterior axis is
specified by mesoderm. 110 589-607.
Frohman MA, Downs TR, Chomczynski P, Frohman LA (1989). Cloning
and characterization of mouse growth hormone-releasing hormone (GRH)
complementary DNA: increased GRH messenger RNA levels in the growth
hormone-deficient lit/lit mouse. Molecular endocrinology (Baltimore, Md.) 3 1529-36.
Frohman, M.A. and Martin, G.R. (1989). Rapid
amplification of cDNA ends using nested primers 1 165-170.
Frohman MA, Martin GR (1989). Cut,
paste, and save: new approaches to altering specific genes in mice. Cell (Cambridge) 56 145-7.
Frohman, M.A (1989). Creating
full-length cDNAs from small fragments of genes: Amplification of rare
transcripts using a single gene-specific oligonucleotide primer. , 28-38..
Frohman, M.A. and Martin, G.R. (1989). Detection
of homologous recombinants. 228-236.
Frohman, M.A. () (1989). Rapid
amplification of cDNA ends using nested primers, a simple and new
technique for cDNA cloning. , .
Martin, G.R. and Frohman, M.A. (1988). The
mouse En-1 gene encodes a nuclear protein expressed in the nervous
system of the developing embryo. NATO AS 43-52.
Frohman MA, Dush MK, Martin GR (1988). Rapid
production of full-length cDNAs from rare transcripts: amplification
using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci U S A. 85 8998-9002.
Wolgemuth DJ, Viviano CM, Gizang-Ginsberg E, Frohman MA, Joyner
AL, Martin GR (1987). Differential
expression of the mouse homeobox-containing gene Hox-1.4 during male
germ cell differentiation and embryonic development. Proc Natl Acad Sci U S A. 84 5813-7.
Francfort JW, Barker CF, Kimura H, Silvers WK, Frohman M, Naji
A (1985). Increased
incidence of Ia antigen-bearing T lymphocytes in the spontaneously
diabetic BB rat. J Immunol. 134 1577-82.
Frohman M, Cowing C (1985). Presentation
of antigen by B cells: functional dependence on radiation dose,
interleukins, cellular activation, and differential glycosylation. J Immunol. 134 2269-75.
Daddona PE, Frohman MA, Kelley WN (1981). Radioimmunoassay
of human adenosine deaminase. Methods Enzymol. 74 Pt C 351-8.
Frohman, L.A., Berilowitz, M., Gonzalez, C., Barowsky, H., Rao,
R., Lim, V.S., Frohman, M.A. and Thominet, J.L. (1981). Studies
of dopaminergic mechanisms in hyperprolactinemic states Vol. 2 39-52..
Daddona PE, Frohman MA, Kelley WN (1980). Human
adenosine deaminase and its binding protein in normal and adenosine
deaminase-deficient fibroblast cell strains. J Biol Chem. 255 5681-7.
Daddona, P.E., Frohman, M.A. and Kelley, W.N. (1980).
Radioimmunochemical analysis of human erythrocyte adenosine deaminase. 3 B.
Frohman MA, Frohman LA, Goldman MB, Goldman JN (1979). Use
of protein A--containing Staphylococcus aureus as an immunoadsorbent in
radioimmunoassays to separate antibody-bound from free antigen. 93 614-21.
Daddona PE, Frohman MA, Kelley WN (1979). Radioimmunochemical
quantitation of human adenosine deaminase. Journal of clinical investigation 64 798-803.
Daddona PE, Frohman MA, Kelley WN (1979). Radioimmunochemical
analysis of human erythrocyte adenosine deaminase. Adv Exp Med Biol. 122B 157-62.
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