Research Interests: Insulin-stimulated translocation of the GLUT4 glucose transporter
Publications
Lodhi I.J., Chiang S.H., Chang L., Vollenweider D., Watson R.T., Inoue M., Pessin J.E., Saltiel
A.R. (2007). Gapex-5, a Rab31
guanine nucleotide exchange factor that regulates Glut4 trafficking in
adipocytes. Cell Metab. 5:59-72.
Hou,
J.C., Suzuki, N., Pessin, J.E., and Watson, R.T (2006). A Di-leucine Motif is Required for the
GGA-dependent Entry of Newly-synthesized IRAP into the Insulin-responsive GLUT4
Storage Compartment. J Biol Chem.
281:33457-66.
Liu,
G., Hou, J.C., Watson, R.T.,
and Pessin, J.E. (2005). Initial entry of IRAP into the insulin-responsive
storage compartment occurs prior to basal or insulin-stimulated plasma membrane
recycling. Am J Phyiol: Endocrinology and
Metabolism. 289:E746-52.
Khan, A.H., Capilla, E., Hou, J.C., Watson, R.T., Smith, J.R., and Pessin, J.E. (2004). Entry of
newly synthesized GLUT4 into the insulin-responsive storage compartment is
dependent upon both the amino terminus and the large cytoplasmic loop. J
Biol Chem 279:37505-11.
Watson,
R.T., Khan, A.H., Furukawa, M.,
Hou, J., Li, L., Kanzaki, M., Okada, S., Kandror, K.V., Pessin, J.E. (2004).
Entry of newly-synthesized GLUT4 into the insulin-responsive storage
compartment is GGA-dependent. EMBO J. 23:2059-2070.
Shigematsu
S., Watson R.T., Khan A.H.,
Pessin, J.E. (2003).The adipocyte plasma membrane caveolin
functional/structural organization is necessary for the efficient endocytosis
of GLUT4. J Biol Chem 278:10683-10690.
Watson,
R.T., Furukawa, M., Chiang,
S.-H., Boeglin, D., Kanzaki, M., Saltiel, A.R., Pessin, J.E. (2003). The
exocytotic trafficking of TC10 occurs through both classical and nonclassical
secretory transport pathways in 3T3L1 adipocytes. Mol Cell Biol. 23:961-974.
Carey,
K.D., Watson, R.T, Pessin
J.E., and Stork, P.J.S. (2003). The requirement of specific membrane domains
for Raf-1 phosphorylation and activation. J Biol Chem 278:3185-3196.
Kanzaki,
M., Watson, R.T., Hou, J.C.,
Stamnes, M., Saltiel, A.R., Pessin, J.E. (2002). The small GTP binding protein
TC10 differentially regulates two distinct populations of filamentous actin in
3T3L1 adipocytes. Mol Biol Cell
13:2334-46.
Kanzaki,
M., Watson, R.T., Khan, A.,
Pessin, J. E. (2001). Insulin stimulates actin comet tails on intracellular
GLUT4-containing compartments in differentiated 3T3L1 adipocytes. J Biol Chem 276: 49331-49336.
Watson, R.T., Shigematsu,
S., Chiang, S.-Y., Mora, S., Kanzaki, M., Macara, I.G., Saltiel, A.R., Pessin,
J.E. (2001). Lipid raft compartmentalization of TC10 is required for insulin
signaling and GLUT4 translocation. J Cell Biol 154:829-840.
Watson,
R.T. and Pessin, J.E. (2001). Transmembrane
domain length determines intracellular membrane compartment localization of
syntaxins 3, 4, and 5. Am J Physiol
Cell Physiol 281:C215-C233.
Chiang,
S.-H., Baumann, C.A., Kanzaki, M., Thurmond, D.C., Watson, R.T., Neudauer, C.L., Macara, I.A., Pessin, J.E.,
Saltiel, A.R. (2001). Insulin-stimulated translocation requires the
CAP-dependent activation of the small GTP binding protein TC10. Nature 410:944-948.
Watson, R.T. and Pessin, J.E. (2000). Functional cooperation
of two independent targeting domains in syntaxin 6 is required for its
efficient localization in the trans-Golgi network of 3T3L1 adipocytes. J Biol Chem 275:1261-1268.
Yang, C.M., Watson, R.T., Elmendorf, J.S., Sacks, D.S., Pessin, J.E.
(2000). Calmodulin antagonists inhibit insulin-stimulated GLUT4 (glucose
transporter 4) translocation by preventing the formation of phosphatidylinositol
3,4,5-trisphophate in 3T3L1 adipocytes. Mol Endocrinol 14:317-326.
Kanzaki, M., Watson, R.T., Artemyev, N.O., Pessin. J.E. (2000). The
trimeric GTP-binding protein (Gq/G11) a subunit is required for
insulin-stimulated GLUT4 translocation in 3T3L1 adipocytes. J Biol Chem 275:7167-7175.
Review Articles/Book
Chapters
Watson,
R.T. and Pessin, J.E. (2007). ‘Glucose’,
in The McGraw-Hill Encyclopedia of Science &
Technology, 10th Edition (Submitted).
Watson,
R.T. and Pessin, J.E. (2007).
GLUT4 translocation: The last 200 nanometers. Cellular Signalling (In Press).
Watson,
R.T. and Pessin, J.E. (2006).
Bridging the GAP between insulin signaling and GLUT4 trafficking. Trends in
Biochem Sci. 31:215-22.
Watson, R.T., Saltiel, A.R., Pessin J.E., Kanzaki, M. (2005).
Subcellular compartmentalization of insulin signaling processes and GLUT4
trafficking events. In: Mechanisms of Insulin
Action, Edited by Drs. Alan Saltiel and
Jeffrey Pessin. Eurekah Bisocience 1:602-612.
Watson,
R.T., Kanzaki, M., Pessin, J.E.
(2004). Regulated membrane trafficking of the insulin-responsive glucose
transporter 4 in adipocytes.Endocr
Rev. 25:177-204.
Watson, R.T. and Pessin, J.E. (2001) Subcellular
compartmentalization and trafficking of the insulin-responsive glucose
transporter, GLUT4. Exp Cell Res
271:75-83.
Watson, RT and Pessin JE. (2001) Intracellular organization of insulin signaling and GLUT4 translocation. Recent Prog Horm Res. 56:175.
