Insertion of tags into existing or new expression vectors
We can introduce HA, His, Flag tags by PCR or add fluorescent protein tag by sub-cloning.
Subcloning from target plasmid provided by investigator into new vector
Cut and paste method or using PCR.
Assistance by consultation to retrieve target cDNA
from Invitrogen cDNA library resource
Point mutation
Using site-directed mutagenesis or "round the horn PCR"
Pilot studies for protein expression and solubility
Protein induction in bacterial expression systems,
gel electrophoresis and coomassie blue staining.
Plasmid transformation into commonly used bacterial cell strains
DH5a, BL21-DE3, XL1-Blue and XL-gold
Competent cells available
On fee for service basis
Typical times required for completion of services
Time will depend on complexity of the project as
discussed during initial consultation.
simple cut and paste subcloning can be completed
within a week.
mutagenesis 4-6 days after receiving primers.
Sequence verification analysis
As requested
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