Matthew J. Camiolo
B.S. Cornell University, 2004

2nd Year Graduate Student

Advisor: Raffaella Sordella, Ph.D.

Department: Cold Spring Harbor Laboratory

Graduate Program: Genetics

Abstract (rotation):

Advisor: Dr. Raffaella Sordella, Cold Spring Harbor Laboratory

Title: Detection and Characterization of Progenitor Cells in Non-Small Cell Lung Carcinoma

Matthew Camiolo, Kannanganattu V. Prasanth, Grace Chan, Laurence Denis, Tetsuya Nakamura and David L. Spector

Lung cancer is currently the leading cause of cancer mortality worldwide. Although improved methods of surgical resection have helped increase 1-year survival rates from time of diagnosis, the 5-year rate for all stages remains a rather dismal number. One possible explanation for both the recurrence of solid tumors after surgical debridement and the emergence of drug resistance following chemotherapy is that these traditional therapies fail to target a population of cells within the tumor that display progenitor or stem-cell like properties. These "cancer stem cells" may hold the key to understanding some of the major road blocks to extending 5-year survival in lung as well as other carcinomas. Using magnetic assisted cell sorting, we have been able to show that enrichment or depletion of cultured cells for a rare population expressing specific surface markers effects clonogenecity and survival in subsequent passaging. Using FACS analysis, we are able to show that this population is present in several non-small cell lung carcinoma cell lines. Finally, using fluorescent marker lineage tracing, we are able to show that depletion of a heterogeneous population for cells expressing this surface marker impacts the effect of the population to survive and repopulate after cytotoxic therapy.

Publications:
(MSTP-supported publications indicated with an *)

7SK RNA IS A RIBOREGULATOR FACILITATING THE DISSOCIATION OF THE pTEF-B COMPLEX FROM TRANSCRIPTION SITES.
Matthew Camiolo, Kannanganattu V. Prasanth, Grace Chan, Laurence Denis, Tetsuya Nakamura and David L. Spector Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, U.S.A.

NUCLEAR RETAINED RNAS REGULATING GENE EXPRESSION
Kannanganattu V. Prasanth, Supriya G. Prasanth, Zhenyu Xuan, Hongjae Sunwoo, Matthew Camiolo, Michael Zhang and David L. Spector Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, U.S.A.