Faculty / Research

Feng-Qian Li, PhD

Associate Professor of Research 

 

Ph.D., Graduate University for Advanced Studies, Japan

Postdoctoral, Dept. of Medicine, University of Washington

(631) 638-1285  feng-qian.li@stonybrook.edu
Signaling: Cell Signaling, Cancer, Stem cells, Protein trafficking and Disease

We are interested in many aspects of developmental, cellular and molecular biology. Currently, we focus on studying the function of signaling regulators involved in cell growth regulation and cancer biology. One of the major interests in the laboratory is to decipher cellular and molecular mechanisms by which Chibby, the antagonist of β-catenin, modulates signal transduction pathways, particularly, the Wnt/β-catenin pathway, thereby effects expression of downstream target genes and influences important cellular events. Our exciting projects involve the analysis of intracellular signaling pathways using conditional gene knockout (Cre-LoxP) approach in mice, and are complimented with in vitro cell culture and biochemical analysis. 

Research Opportunities
Please contact Feng-Qian Li at feng-qian.li@stonybrook.edu or 631-638-1285 for information regarding the availability of post-doctoral, graduate and undergraduate positions.
Selected publications

Siller, S. S., Burke, C. M., Li, F.-Q., & Takemaru, K.-I. (2015)
Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells.
Cell Cycle. Aug 12:0. PMID: 26266958

Li, F.-Q., Siller, S. S., & Takemaru, K.-I. (2015).
Basal body docking in airway ciliated cells. Oncotarget, Vol. 6, No. 24

Burke, M.C., Li, F.-Q., Cyge, B., Arashiro, T., Brechbuhl, H. M., Chen X. W., Siller, S. S., Weiss M. A., O’Connell C. B., Love, D., Westlake C. J., Reynolds, S. D., Kuriyama. R. & Takemaru, K.-I. (2014)
Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.
J. Cell Biol., 207 (1), 123-137 PMCID: PMC4195830
• Featured on Journal Cover and highlighted in “In This Issue”.

Lee, Y.-L., Sante, J., Comerci C. J., Cyge, B., Menezes L. F., Li, F.-Q., Germino G. G., Moernerr W. E.,  Takemaru, K.-I., & Stearns, T. (2014)
Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function.
Mol. Biol. Cell, 25(19): 2919-2933. PMCID: PMC4230582

Steere, N,, Chae, V., Burke, M., Li, F.-Q., Takemaru, K.-I., & Kuriyama, R. (2012)
A Wnt/β-catenin pathway antagonist Chibby binds Cenexin at the distal end of mother centrioles and functions in primary cilia formation.
PLoS ONE, 7(7): e41077. PMCID: PMC3401179

Fischer, V., Brown-Grant, D.-A., & Li, F.-Q. (2012)
Chibby suppresses growth of human SW480 colon adenocarcinoma cells through inhibition of β-catenin signaling.
J. Mol. Signal., 31;7(1):6. PMCID: PMC3463480

Takemaru, K.-I.,, Chen, X.W. and & Li, F.-Q. (2012)
β-Catenin.
Encyclopedia of signaling molecules, Springer


Cyge, B., Fischer, V., Takemaru, K.-I., & Li, F.-Q., (2011)
Generation and characterization of monoclonal antibodies against human Chibby protein.
Hybridoma, 30 (2):163-168. PMCID: PMC3133832

Cyge, B., Fischer, V., Takemaru, K.-I., & Li, F.-Q. (2011)
Mouse anti-Chibby monoclonal antibodies (clones 8-2 and 27-11).
Hybridoma. 30 (2) 208. PMID: 21529299

Love, D., Li, F.-Q., Burke, M.C., Cyge, B., Ohmitsu, M., Cabello, M., Larson, J.E., Brody, S.L., Cohen, J.C., & Takemaru, K.-I. (2010)
Altered lung morphogenesis, epithelial cell differentiation and mechanics in mice deficient in the Wnt/β-catenin antagonist Chibby.
PLoS One, 5(10): e13600. doi:10.1371/journal.pone.0013600. PMCID: PMC2963606

*Co-correspondence
*Li, F.-Q., Mofunanya, A., Fischer, V., Hall, J., & *Takemaru, K.-I. (2010)
Nuclear-cytoplasmic shuttling of Chibby regulates subcellular localization and signaling activity of β-catenin.
Mol. Biol. Cell, 21:311-322. PMCID: PMC2808236

Mofunanya, A., Li, F.-Q., Hsieh, J.-C. & Takemaru, K.-I. (2009)
Chibby forms a homodimer through a heptad repeat of leucine residues in its C-terminal coiled-coil motif.  
BMC Mol. Biol., 10:41 doi: 10.1186/1471-2199-10-41. PMCID: PMC2686680

Voronina, V.A., Takemaru, K.-I., Treuting, P.,  Love, D., Grubb, B.R., Hajjar, A.M.,  Adams, A.,  Li, F.-Q., & Moon, R.T. (2009)
Inactivaton of Chibby affects function of motile airway cilia.
J. Cell Biol., 185 (2): 225-233. PMCID: PMC2700371

*Co-correspondence
Takemaru, K.-I., Fischer, V., & Li, F.-Q. (2009)
Fine-tuning of nuclear β-catenin by Chibby and 14-3-3.
Cell Cycle. 8 (2): 8: 210-213. PMCID: PMC2810615
*Li, F.-Q., Mofunanya, A., Harris, K., & *Takemaru, K.-I. (2008)
Chibby cooperates with 14-3-3 to regulate β-catenin subcellular distribution and signaling activity.
J. Cell Biol., 181 (7): 1141-1154. PMCID: PMC2442201
•  Research Highlights in Nature Reviews-Molecular Cell Biology by Kritikou, (2008)- (http://www.nature.com/nrm/journal/v9/n8/full/nrm2462.html), Nat. Rev. Mol. Cell Biol., 9:584.
•  Selected Updates in Nature Signaling Gateway  (2008)-AKTing in Wnt pathway (http://www.signaling-gateway.org/update/updates/200808/nrm2462.html).
•  Faculty of 1000 Cell Biology (2008) by Frans Van Roy -(http://www.f1000biology.com/article/id/1122828/evaluation)

Takemaru, K.-I., Ohmitsu, M, & Li, F.-Q. (2008)
An oncogenic hub: β-catenin as a molecular target for cancer therapeutics.
Handbook Exp. Pharmacology, 186: 261-284. PMID: 18491056

Li, F.-Q., Singh, A.M., Mofunanya, A., Love, D., Terada, N., Moon, R.T., & Takemaru, K.-I. (2007)
Chibby promotes adipocyte differentiation through inhibition of β-catenin signaling.
Mol. Cell Biol., 27 (12): 4347-4354. PMCID: PMC1900052

Singh, A.M., Li, F.-Q., Hamazaki, T., Takemaru, K.-I., & Terada, N. (2007)
Chibby, an antagonist of the Wnt/β-catenin pathway, facilitates cardiomyocyte differentiation of murine embryonic stem cells.
Circulation, 115 (5): 617-626. PMCID: PMC2565513

Benson, K.F., Person, R.E., Li, F.-Q., Williams, K.,  &  Horwitz, M. (2004)
Paradoxical homozygous expression from heterozygotes and heterozygous expression from homozygotes as a consequence of transcriptional infidelity through a polyadenine tract in the AP3B1 gene responsible for canine cyclic neutropenia.
Nucleic. Acids. Res. 32(21): 6327-6333. PMCID: PMC535682

Horwitz, M., Benson, K.F., Duan, Z., Li, F.-Q., & Person R.E. (2004)
Hereditary neutropenia: dogs explain human neutrophil elastase mutations
Trends Mol. Med. 10 (4): 163-170. PMID: 15059607

Li, F.-Q., Person, R.E., Takemaru, K.-I., Williams, K., Meade-White, K., Ozsahin, A.H., Gungor, T., Moon, R.T., & Horwitz, M. (2004)
Lymphoid enhancer factor-1 links two hereditary leukemia syndromes through core-binging factor regulation of ELA2.
J. Biol. Chem. 279 (4): 2873-2884. PMID: 14594802


Duan, Z., Li, F.-Q., Wechsler, J., Meade-White, K., Williams, K., Benson, K.F., & Horwitz, M. (2004)
A novel Notch protein, N2N, targeted by neutrophil elastase and implicated in hereditary neutropenia.  
Mol. Cell Biol. 24 (1): 58-70. PMCID: PMC303357

Benson, K.F., Li, F.-Q., Person, R.E., Albani, D., Duan, Z., Wechsler, J., Meade-White, K., Williams, K., Acland, G.M., Niemeyer, G., Lothrop, C.D., & Horwitz, M. (2003)
Mutations associated with neutropenia in dogs and humans disrupt intracellular transport of neutrophil elastase.  
Nat. Genet. 35(1): 90-96. PMCID: PMC2832179

*Contributed equally
*Person, R.E., * Li, F.-Q., *Duan, Z., Benson, K.F., Wechsler, J., Papadaki, H.A., Eliopoulos, G., Kaufman, C., Bertolone, S.J., Nakamoto, B., Papayannopoulou, T., Grimes, H.L., & Horwitz, M. (2003)
Mutations in proto-oncogene Gfi1 cause human neutropenia and target ELA2.  
Nat. Genet. 34(3): 308-312. PMCID: PMC2832179

Horwitz, M. Benson, K.F., Duan, Z., Person, R.E., Wechsler, J., Williams, K., Albani, D., & Li, F.-Q. (2003)
Role of neutrophil elastase in bone marrow failure syndromes: molecular genetic revival of the chalone hypothesis.
Curr. Opin. Hematol., 10 (1): 49-54. PMID: 12483111

Li, F.-Q., & Horwitz, M. (2001)
Characterization of mutant neutrophil elastase in severe congenital neutropenia.  
J. Biol. Chem., 276 (17): 14230-14241. PMID: 11278653    

Li, F.-Q., Coonrod, A., & Horwitz, M. (2000)
Selection of a dominant negative retinoblastoma protein (RB) inhibiting satellite myoblast differentiation implies an interaction between MyoD and RB.  
Mol. Cell Biol., 20 (14): 5129-5139. PMCID: PMC85962

Takemaru, K.-I., Li, F.-Q., Ueda, H., & Hirose, S. (1997)
Multiprotein bridging factor 1 (MBF1) is an evolutionarily conserved transcriptional coactivator that connects a regulatory factor and TATA element-binding protein.  
Proc. Natl. Acad. Sci. USA, 94 (14): 7251-7256. PMCID: PMC23807

Li, F.-Q., Takemaru, K.-I., Goto, M., Ueda, H., & Hirose, S. (Feb. 1997)
Transcriptional activation through interaction of MBF2 with TFIIA.  
Genes to Cells, 2 (2): 143-153. 143-153. PMID: 9167971

Li, F.-Q., Coonrod, A., & Horwitz, M. (1996).
Preferential MyoD homodimer formation demonstrated by a general method of dominant negative mutation employing fusion with a lysosomal protease.  
J. Cell Biol., 135 (4): 1043-1057. PMCID: PMC2133387

Li, F.-Q., Ueda, H., & Hirose, S. (1994).
Mediators of activation of fushi tarazu gene transcription by BmFTZ-F1.  
Mol. Cell. Biol., 14 (5): 3013-3021. PMCID: PMC358669.

Dept. of Pharmacology BST 7-186/183
Stony Brook, NY 11794-8651
Phone: (631) 638-1285
Fax: (631) 444-3218
E-mail: feng-qian.li@stonybrook.edu