Nicole S. Sampson, Ph.D. ()

Nicole
S.
Sampson
Ph.D.

Ph.D., 1990, University of California, Berkeley
American Cancer Society Postdoctoral Fellowship, Harvard University, 1991-1993.

Protein Structure and Function
Overview

Our research program focuses on proteins. How do they effect catalysis? How does the interaction of two or more proteins initiate a biological process? The methodologies utilized in our laboratory comprise a broad range of techniques; they include organic synthesis, enyzme kinetics, NMR spectroscopy and molecular biology.

 

Work is ongoing in three different areas:

  1. Study of catalysis
    and binding of steroids
  2. Study of protein
    hinges
  3. Investigation
    of proteins involved in adhesion and signalling during mammalian fertilization
  • Chen, H.; Sampson, N.S., (1999)
    "Mediation of Mammalian Sperm-Egg Fusion: Evidence That Mouse Egg a6b1 Integrin
    is the Receptor for Sperm Fertilinb," Chem. Biol., 6, 1-10.

 

  • Yue, Q. K.; Kass, I. J.; Sampson,
    N. S.; Vrielink, A., (1999) "Crystal Structure Determination of Cholesterol
    Oxidase from Streptomyces and Structural Characterization of Key Active Site
    Mutants," Biochemistry, 38, 4277-4286.

 

  • Sun, J.; Sampson, N. S., (1999)
    "Understanding Protein Lids: Kinetic Analysis of Active Hinge Mutants in Triosephosphate
    Isomerase," Biochemistry, 38, 11474-11481.

 

  • Gupta, S.; Li, H.; Sampson, N. S.,
    (2000) "Characterization of Fertilinb-Disintegrin Binding Specificity in Sperm-Egg
    Adhesion," Bioorg. Med. Chem., 8, 723-729.

 

  • McCann, A.; Sampson, N. S., (2000)
    "A C6-FAD Adduct is Formed Upon Irreversible Inactivation of Cholesterol Oxidase
    by 2a,3a-Cyclopropano-5-cholestan-3b-ol," J. Am.Chem. Soc., 122, 35-39.

 

  • Chen, X.; Wolfgang, D.; Sampson,
    N. S., (2000) "Use of the Parallax-Quench Method to Determine the Position of
    the Active-Site Loop of Cholesterol Oxidase in Lipid Bilayers," in press, Biochemistry.

 

  • Sampson, N. S.; Sarah T. Ryan; Deborah
    A. Enke; Dominic Cosgrove; Victor Koteliansky; Philip Gotwals (2000) "Identification
    of a Role for a1Integrin in Alport Syndrome using Global Analysis of Gene Expression,"
    submitted to Nature Medicine.